Spectrophotometric Assay of Cefadroxil via Oxidation and Bleaching Color of Leishman Dye

Othman, Nabeel Sabeeh; Suliaman, Amina Daoud

doi:10.18576//jpac.2023.210104.1010

Spectrophotometric Assay of Cefadroxil via Oxidation and Bleaching Color of Leishman Dye

Document Type : Original Article

Authors

¹ chemistry /college of science/ university of Mosul

² chemistry/science/Mosul

10.18576//jpac.2023.210104.1010

Abstract

Cefadroxil is a drug used in many treatments of infections resulting mainly from Gram-positive bacteria. A simple and sensitive spectrophotometric method has been developed for the estimate of cefadroxil in the pure form and in the dosage form(capsule). The suggested method involves the oxidation of cefadroxil with a known excess amount of N-bromosuccinimide (NBS) in an acidic medium (hydrochloric acid, 1M). The residual amount of oxidant (NBS) is determined by decolorization of the Leishman color dye. The absorbance of excess dye was measured at 651 nm. The method follows Beer's law in the concentration range of 0.5-7.0 µg/ml. The linearity with determination coefficient 0.9941, molar absorptivity 3.5813 x 104 L.mol-1.cm-1 and Sandellʹs sensitivity index value 0.0106 µg/cm2. The LOD and LOQ values were 0.004 μg/ml and 0.015 μg/ml respectively. The relative standard deviation value was not more than 4.88% and the relative error was from -2.0 to 1.3%. The method was applied successfully to the pharmaceutical preparations as a capsule. a capsule.

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