Inhibition of Acetylcolinesterase Enzyme from Pegagan Herb Extract (Centella asiatica), Kelor Leaf (Moringa oleifera) Extract and Combinations

Tri Atmodjo Reubun, Yonathan; Kumala, Shirly; Setyahadi, Siswa; Simanjuntak, Partomuan

doi:10.18576/jpac/070104

Inhibition of Acetylcolinesterase Enzyme from Pegagan Herb Extract (Centella asiatica), Kelor Leaf (Moringa oleifera) Extract and Combinations

Document Type : Original Article

Authors

¹ 1Faculty of Pharmacy, Pancasila University, Lenteng Agung Srengseng Sawah, Indonesia 2Center for Bioindustrial Technology, Agency for the Assessment and Application of Technology, Indonesia 3Center for Biotechnology, Indonesian Institute of Sciences, Bogor, Jawa Barat, Indonesia

² Faculty of Pharmacy, Pancasila University, Lenteng Agung Srengseng Sawah, Indonesia

³ Center for Bioindustrial Technology, Agency for the Assessment and Application of Technology, Indonesia

⁴ Center for Biotechnology, Indonesian Institute of Sciences, Bogor, Jawa Barat, Indonesia

10.18576/jpac/070104

Abstract

Alzheimer's disease (AD) is a disease in which brain damage is characterized by decreased attention, memory,
and personality. AD is one result of impaired acetylcholine function. In this case, acetylcholinesterase (AChE) is an
enzyme that functions as a catalyst for the breakdown of acetylcholine (ACh) into inactive forms acetate and choline. The
purpose of this study was to determine the treatment activity of AD through AChE inhibition by Centella asiatica extract,
Moringa oleifera extract and both of combinations. The activity test was carried out based on the Ellman method wherein
this method was based on the hydrolysis ATCh substrate reaction by AChE with DTNB which gave a yellow color and
measured its absorption at a wavelength of 410 nm. The results of the AChE inhibition test showed that the combination of
pegagan herbs extract and kelor leaf extract 1: 1 gave the best inhibition with an IC50 value of 217.588 ppm. Meanwhile,
Eserin positive control with an IC50 value of 5.010 ppm. The combination of Centella asiatica extract and Moringa oleifera
extract has a synergistic effect on the treatment of AD.

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