Validated HPLC Method for Simultaneous Determination of Aripiprazole and Co-Administered Clonazepam in Spiked Human Plasma

M. El-Maraghy, Christine; Salem, Hesham; M. Amer, Sawsan; Nebsen, Marianne

doi:10.18576/jpac/030108

Validated HPLC Method for Simultaneous Determination of Aripiprazole and Co-Administered Clonazepam in Spiked Human Plasma

Document Type : Original Article

Authors

¹ Analytical Chemistry department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 11787 6th October city, Egypt.

² Pharmaceutical Analytical Chemistry department, Faculty of Pharmacy, Deraya University, Minia, Egypt.

³ Analytical Chemistry department, Faculty of Pharmacy, Cairo University, Kasr-El Aini Street, 11562Cairo, Egypt.

⁴ 3Analytical Chemistry department, Faculty of Pharmacy, Cairo University, Kasr-El Aini Street, 11562Cairo, Egypt. 4Pharmaceutical Analytical Chemistry department, Faculty of Pharmacy & Drug Technology, Heliopolis University, 3 Cairo Belbeis desert road, 2834El- Horria, Cairo, Egypt.

10.18576/jpac/030108

Abstract

A sensitive and selective high performance liquid chromatographic method was developed and validated for the simultaneous quantification of Aripiprazole and co-administered Clonazepam in spiked human plasma. Aripiprazole, Clonazepam and the internal standard Citalopram were extracted from plasma and analyzed on a reversed-phase C18 column using a mobile phase consisting of acetonitrile and 0.05M potassium dihydrogen orthophosphate (pH= 3.6) in a ratio of (40: 60, v/v). The ultraviolet (UV) detection was at 220 nm. The method was linear over concentration range (20-1000 ng mL-1) for both drugs. The method is very simple and allows obtaining a very good recovery of the analytes.

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