Relationship of C-Reactive Proteins with Oxidative Stress and Obesity in Type 2 Diabetic Subjects

Document Type : Original Article


1 Department of Chemistry, Faculty of Sience, Sohage University, Sohag 82524, Egypt.

2 Zoology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt.

3 Department of Internal Medicine, Faculty of Medicine, Sohag University, Sohag 82524, Egypt.



The present work was aimed to study the correlation between C-reactive proteins as marker of acute phase response, total antioxidants capacity as marker of oxidative stress and blood pictures with the electrophoresis patterns of serum protein in sera of subjects with obese and diabetic obese subjects attending Sohag University Hospital.
Subjects: The total number of study subjects was 85 subjects, 30 (35.2%) individuals with BMI 20.11 were control, 31(36.4%) subjects with BMI 31.99 were obese and 24 (28.2%) subjects with BMI 30.62 were obese diabetic type 2.
Method: In sera, C-reactive protein and immunoglobulin G was assayed by ELISA protocol, total antioxidant capacity was measured by colorimetric method, blood pictures were analyzed by haematological analyzer (Celldyne 3700) and sera proteins fractions were analyzed by electrophoresis.
Results: The present study was found that the counts of lymphocytes, monocytes and platelets were increased in non-diabetic obese and diabetic obese subjects compared to controls but still in normal range, red blood cells and hematocrite were decreased compared to control and still in the normal range, C-reactive protein was significantly elevated, however, total antioxidant capacity was decreased in non-diabetic obese and diabetic obese subjects compared to controls. In addition, immunoglobulines in electrophoretic pattern was increased compared to control.
Conclusion: The present results confirm the positive correlation between the acute phase response of inflammation and oxidative stress in non-diabetic and diabetic obese subjects.
Keywords: Obesity, type 2 Diabetes, oxidative stress, IgG, TAC, CRP. Albumin, Globulins.